Applied Biochemistry and Biotechnology 2001-01-01

Characterization of DNA immobilized on electrochemically prepared conducting polypyrrole-polyvinyl sulfonate films.

A Gambhir, M Gerard, S K Jain, B D Malhotra

Index: Appl. Biochem. Biotechnol. 96(1-3) , 303-9, (2001)

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Abstract

The article describes the adsorption characteristics of DNA onto electrochemically generated polypyrrole-polyvinyl sulfonate (PPY-PVS) films obtained as a function of pH. Adsorption on PPY doped with an anion proceeds by anion exchange, and since DNA possesses a fixed negative charge owing to PO4-, it favors a very strong binding displacing PVS with favorable energetic interactions. Characterization of adsorbed DNA onto the PPY-PVS films was carried out by ultraviolet-visible, Fourier transform infrared spectroscopy, and cyclic voltammetric studies.