Biochimica et Biophysica Acta 1997-03-28

Genetic evidence for the existence of two quinone related inhibitor binding sites in NADH-CoQ reductase.

E Darrouzet, A Dupuis

Index: Biochim. Biophys. Acta 1319(1) , 1-4, (1997)

Full Text: HTML

Abstract

Using the NADH-CoQ reductase of Rhodobacter capsulatus as a model for the mitochondrial Complex I, we have for the first time isolated bacterial mutants resistant to piericidin-A, a classical inhibitor of the mitochondrial enzyme. Their sensitivity to other inhibitors directed towards the quinone binding domain of complex I gives direct genetic evidence for the existence of two inhibitor binding sites.