Designing new Baeyer-Villiger monooxygenases using restricted CASTing

CM Clouthier, MM Kayser, MT Reetz

Index: Clouthier, Christopher M.; Kayser, Margaret M.; Reetz, Manfred T. Journal of Organic Chemistry, 2006 , vol. 71, # 22 p. 8431 - 8437

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Citation Number: 89

Abstract

This paper outlines the design and execution of the first mini-evolution of cyclopentanone monooxygenase (CPMO). The methodology described is a relatively inexpensive and rapid way to obtain mutant enzymes with the desired characteristics. Several successful mutants with enhanced enantioselectivities were identified. For example, mutant-catalyzed oxidation of 4-methoxycyclohexanone gave the corresponding lactone with 92% entantiometric ...