Analytical Biochemistry 1998-12-15

Preparative purification of a high-mannose type N-glycan from soy bean agglutinin by hydrazinolysis and tyrosinamide derivatization.

D L Evers, R L Hung, V H Thomas, K G Rice

Index: Anal. Biochem. 265(2) , 313-6, (1998)

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Abstract

The N-linked oligosaccharide from soy bean agglutinin (Man9) was isolated on a preparative scale following derivatization with Boc-tyrosine. The procedure utilized preparative hydrazinolysis to release the oligosaccharide and yielded multi-micromol quantities of Boc-tyrosine-Man9 which was characterized by 1H NMR and ES-MS.Copyright 1998 Academic Press.