Bioorganic & Medicinal Chemistry 2005-07-15

Mechanism and structure-activity relationships of norspermidine-based peptidic inhibitors of trypanothione reductase.

Mark J Dixon, Richard I Maurer, Cristina Biggi, Julen Oyarzabal, Jonathan W Essex, Mark Bradley

Index: Bioorg. Med. Chem. 13(14) , 4513-26, (2005)

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Abstract

A library of polyamine-peptide conjugates based around some previously identified inhibitors of trypanothione reductase was synthesised by parallel solid-phase chemistry and screened. Kinetic analysis of library members established that subtle structural changes altered their mechanism of action, switching between competitive and non-competitive inhibition. The mode of action of the non-competitive inhibitors was investigated in detail by a variety of techniques including enzyme kinetic analysis (looking at both NADPH and trypanothione disulfide substrates), gel filtration chromatography and analytical ultracentrifugation, leading to the identification of an allosteric mode of inhibition.