Xenobiotica 2014-08-01

Glucuronidation of aurantio-obtusin: identification of human UDP-glucuronosyltransferases and species differences.

Bao-Li Mi, Qi Sun, Yan-Qing Qu, Xiao-Xu Gao, Zhen-Wen Yu, Guang-Bo Ge, Shan-Shan Cai, Jie Zhang, Yan-Chao Zheng, Zhen-Qiu Zhang

Index: Xenobiotica 44(8) , 716-21, (2014)

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Abstract

1. The aurantio-obtusin's glucuronide was detected when aurantio-obtusin was incubated with human liver microsomes (HLMs). Recombinant UGT isoforms screening experiment showed that UGT1A8 was the major isoform contributed to the glucuronidation. 2. The metabolic profiles for aurantio-obtusin in liver microsomes from different species were similar, however, the intrinsic clearance values (Vmax/Km) among the species were: Monkey > Human > Rat > Rabbit > Dog > Pig > Mouse > Guinea pig.