Methods in Molecular Biology 2012-01-01

Reconstitution of a secondary metabolite biosynthetic pathway in a heterologous fungal host.

Takayuki Itoh, Tetsuo Kushiro, Isao Fujii

Index: Methods Mol. Biol. 944 , 175-82, (2012)

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Abstract

Expression of multiple genes involved in a particular metabolic pathway in a heterologous host facilitates the study of fungal secondary metabolite biosynthesis and production of useful compounds. Two plasmids with different selection markers, argB and the pyrithiamine resistance marker, are used to transform Aspergillus oryzae allowing for expression of up to three genes simultaneously.