Rapid Communications in Mass Spectrometry 2006-01-01

Identification of isocephalomannine in the presence of cephalomannine isomers and alkali metal ion adducts in a paclitaxel active pharmaceutical ingredient using electrospray tandem mass spectrometry.

Kannan Vivekanandan, Madugundu Guru Swamy, Sudhanand Prasad, Rama Mukherjee, Anand C Burman

Index: Rapid Commun. Mass Spectrom. 20(11) , 1731-5, (2006)

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Abstract

A strategy is developed for the identification of isocephalomannine in the presence of alkali metal ion adducts and other cephalomannine isomers in a paclitaxel active pharmaceutical ingredient. Intact molecular ion analyses and a sub-structural study have been performed for the differentiation of isocephalomannine (2-debenzoylpaclitaxel-2-pentenoate) from cephalomannine and 7-epi-cephalomannine. A comparative study of the cephalomannine isomers was carried out using molecular ions (MS) and fragmentation patterns (MS/MS) for sub-structural analysis. An attempt has been made to identify isocephalomannine in Cremophor(R) EL formulations.Copyright (c) 2006 John Wiley & Sons, Ltd.