Journal of Steroid Biochemistry 1989-02-01

Prevention of bridge binding in immunoassays: a general estradiol tracer structure.

L X Tiefenauer, D M Bodmer, W Frei, R Y Andres

Index: J. Steroid Biochem. 32(2) , 251-7, (1989)

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Abstract

Iodinated estradiol tracers were synthesized with three different bridges connecting the radiolabelled moiety to the steroid core: Hemisuccinate, carboxymethyloxime and amide. Taking these iodinated tracers in combination with ten antibodies raised against estradiol-6-CMO-albumin, titers and slopes of calibration curves have been compared to the corresponding data using a 3H tracer. The data indicate that the tracer with the amide bridge is recognized similarly to the tritiated estradiol by all antibodies tested, whereas the two other iodinated tracers exhibit substantial bridge binding. The results suggest that the amide tracer structure can generally be used to improve the quality of estradiol antibodies suffering from bridge binding effects.