PNAS 1976-06-01

Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.

W L McKeehan, W G Hamilton, R G Ham

Index: Proc. Natl. Acad. Sci. U. S. A. 73(6) , 2023-7, (1976)

Full Text: HTML

Abstract

The trace element selenium is essential for clonal growth of diploid fibroblasts from human fetal lung (WI-38) in media containing small amounts of serum protein. Maximum growth stimulation is obtained when 30 nM neutralized selenious acid is added to a synthetic medium containing 1.5 mg/ml of dialyzed fetal bovine serum protein (equivalent to a 3% serum concentration). Serum appears to be a source of selenium in most culture media, since higher concentrations of serum protein or whole serum mask the selenium requirement of WI-38 cells. Selenium is also required by a Chinese hamster cell line that can be grown in a protein-free synthetic culture medium.