Analytical Biochemistry 1987-08-15

2,3-Diaminophenazine is the product from the horseradish peroxidase-catalyzed oxidation of o-phenylenediamine.

P J Tarcha, V P Chu, D Whittern

Index: Anal. Biochem. 165 , 230, (1987)

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Abstract

NMR and mass spectroscopic evidence has been obtained which indicates that the product of the oxidation of o-phenylenediamine by hydrogen peroxide, uncatalyzed or catalyzed by horseradish peroxidase, is 2,3-diaminophenazine. These results settle disparate literature descriptions. The process is most likely free radical in nature starting with the abstraction of a labile amino hydrogen atom.