Journal of Biotechnology 2011-03-10

Site-specific tetrameric streptavidin-protein conjugation using sortase A.

Takuya Matsumoto, Shiori Sawamoto, Takayuki Sakamoto, Tsutomu Tanaka, Hideki Fukuda, Akihiko Kondo

Index: J. Biotechnol. 152 , 37-42, (2011)

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Abstract

Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and streptavidin modification of proteins or small molecules is widely used for biotechnology tool. Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We focused on sortase A, a transpeptidase from Staphylococcus aureus. A streptavidin-tagged LPETG motif (Stav-LPETG) was expressed in Escherichia coli. We achieved soluble streptavidin expression in E. coli without refolding using a cold shock expression system. Then we successfully conjugated Stav-LPETG with pentaglycine-appended green fluorescence protein (Gly5-GFP) or triglycine-appended glucose oxidase (Gly3-GOD) using sortase A. SDS-PAGE analysis showed site-specific tetrameric streptavidin-protein conjugation with the tagged proteins. In addition, the functions of a Stav-GOD conjugate, i.e., biotin-binding and glucose oxidase activity, were significantly higher compared to those of streptavidin-GOD conjugates prepared by chemical modification.Copyright © 2011 Elsevier B.V. All rights reserved.