Journal of Bioscience and Bioengineering 2005-09-01

Hyper expression of kojibiose phosphorylase gene and trehalose phosphorylase gene from Thermoanaerobacter brockii ATCC35047 in Bacillus subtilis and selaginose synthesis utilizing two phosphorylases.

Takuo Yamamoto, Kazuhisa Mukai, Kazuhiko Maruta, Hikaru Watanabe, Hiroshi Yamashita, Tomoyuki Nishimoto, Michio Kubota, Hiroto Chaen, Shigeharu Fukuda

Index: J. Biosci. Bioeng. 100(3) , 343-6, (2005)

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Abstract

The kojibiose phosphorylase (KP) gene and trehalose phosphorylase (TP) gene from Thermoanaerobacter brockii ATCC35047 were intracellularly hyper-expressed under the control of the Bacillus amyloliquefaciens alpha-amylase promoter in Bacillus subtilis. The production yields were estimated to be 2.1 g of KP and 4.9 g of TP per liter of medium. Selaginose, non-reducing trisaccharide, was synthesized from trehalose utilizing the recombinant KP and TP from B. subtilis. Selaginose was not hydrolyzed by salivary amylase, artificial gastric juice, pancreatic amylase, or small intestinal enzymes.