Journal of Microscopy (Oxford) 1981-03-01

A simple method for the use of gallocyanin-chrome alum as an electron strain.

W Sandritter, U Riede, G Kiefer

Index: J. Microsc. 121(Pt 3) , 253-9, (1981)

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Abstract

A method has been elaborated for the demonstration of DNA in the electron microscope. The method uses glutaraldehyde fixed tissue pieces from which RNA has been removed by incubation with RNase. DNA is stained by gallocyanin-chrome alum in the tissue block. Embedding and cutting is done in the usual manner. The method is based on histochemical observations at the light microscope level which show sufficient specificity and a good stoichiometry of the staining reaction.