Acta Histochemica 1981-01-01

[Determination of the relative RNA-content of liver cell nuclei with gallocyanin-chromealum-staining (author's transl)].

K Wenzelides, G Korek, K Voss

Index: Acta Histochem. 69(2) , 307-13, (1981)

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Abstract

The relative RNA-content of liver cell nuclei from histological slides of rat liver was determined by automatical microscope picture analysis. The slides were stained with Gallocyanin-Chromealum. Extinction measurements are made before and after perchloracid extraction of RNA for the same slide. The changes of extinction measurements caused by the changes of slide thickness and of tissue inhomogeneity could be lowered using this method.