Biomedical Chromatography 1990-09-01

Solid phase extraction and high performance liquid chromatographic determination of doxophylline in plasma.

A Lagana, M Bizzarri, A Marino, M Mancini

Index: Biomed. Chromatogr. 4(5) , 205-7, (1990)

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Abstract

A sensitive and selective high performance liquid chromatographic doxophylline assay with ultraviolet detection has been developed for plasma samples. The drug is isolated from biological samples with a reversed phase C18 disposable extraction column. Plasma standard curves are linear for concentrations of doxophylline from 0.03 to 10 mg/L. At the therapeutic range concentrations, the recoveries are better than 96.9%. The coefficients of variation for the procedure are 4.1% and 2.7% for the concentrations 0.03 mg/L and 10 mg/L, respectively. By this method, pharmacokinetic profiles are obtained for six adult volunteers.