FEMS Microbiology Letters 2000-03-15

The utilization of 4-aminobutylphosphonate as sole nitrogen source by a strain of Kluyveromyces fragilis.

N G Ternan, G McMullan

Index: FEMS Microbiol. Lett. 184(2) , 237-40, (2000)

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Abstract

A strain of the yeast Kluyveromyces fragilis was screened for its ability to utilize a range of synthetic and natural organophosphonate compounds as the sole source of phosphorus, nitrogen or carbon. Only 4-aminobutylphosphonate was utilized as sole nitrogen source with protein yields increasing proportionally with substrate concentrations up to 10 mM. No 4-aminobutylphosphonate metabolizing enzyme activity was detectable in cell-free extracts prepared from K. fragilis pregrown on 2.5 mM 4-aminobutylphosphonate. None of the organophosphonates tested served as a source of carbon or phosphorus for K. fragilis.