Journal of Pharmaceutical and Biomedical Analysis 2011-01-05

Determination of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry.

Ping Zhu, Yu-Guan Wen, Ji-Mei Chen, Jian Zhuang, Zhi-Ling Zhou, Shao-Yi Zheng, Ruo-Bin Wu, Xue-Jun Xiao, Cong Lu, Rui-Xin Fan, Hui-Ming Guo, Xiao-Ping Fan

Index: J. Pharm. Biomed. Anal. 54(1) , 198-202, (2011)

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Abstract

A sensitive and rapid method was developed for quantification of olprinone in human plasma utilizing liquid chromatography tandem mass spectrometry (LC-MS/MS). An aliquot of 1 mL plasma sample was extracted with ethyl acetate-dichloromethane. Separation of olprinone and the milrinone (internal standard, IS) from the interferences was achieved on a C(18) column followed by MS/MS detection. The analytes were monitored in the positive ionization mode. Multiple reaction monitoring using the transition of m/z 251 → m/z 155 and m/z 212 → m/z 140 was performed to quantify olprinone and IS, respectively. The method had a total chromatographic run time of 3 min and linear calibration curves over the concentration range of 0.5-60 ng/mL. The lower limit of quantification (LLOQ) was 0.5 ng/mL. The intra- and inter-day precisions were less than 16.3% for low QC level, and 7.1% for other QC levels, respectively. The intra- and inter-day relative errors were ranged between -12.2% and 3.7% for three QC concentration levels. The validated method was successfully applied to the quantification of olprinone concentration in human plasma after intravenous (i.v.) administration of olprinone at a constant rate of infusion of 2 μg/(kg min) for 5 min in order to evaluate the pharmacokinetics.Copyright © 2010 Elsevier B.V. All rights reserved.