Nucleosides, Nucleotides & Nucleic Acids 2011-01-01

Nucleotides part LXXX: Synthesis of 3'-O fluorescence labeled thymidine derivatives and their 5'-O-triphosphates.

Tamara I Prykota, Wolfgang Pfleiderer

Index: Nucleosides Nucleotides Nucleic Acids 30(7-8) , 544-51, (2011)

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Abstract

A new labeling technique attaching a fluorescent pteridine derivative (3, 5) via a linker onto the 3'-OH group of 5'-O-dimethoxytritylthymidine (7) was developed to lead to the conjugates 8 and 11. After detritylation to give 9 and 12, the final conversion into the corresponding 5'-triphosphates (13, 14), which were isolated as sodium salts, was performed by known methods.