Nucleic Acids Research 1982-05-25

Synthesis of mixed oligodeoxyribonucleotides following the solid phase method.

A Elmblad, S Josephson, G Palm

Index: Nucleic Acids Res. 10(10) , 3291-301, (1982)

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Abstract

A method for the synthesis of mixed dimers, trimers and oligonucleotides on a solid support using monomeric protected nucleoside phosphochloridites (1a-d) has been developed and the different nucleoside reagents, and the results show that yields of different oligomers in a mixture could be directly correlated to the concentration of the four reagents. Separation of mixed oligomers on a reversed phase C18 column has also been studied.