Marine Biotechnology 2012-08-01

Detection of bioactive exometabolites produced by the filamentous marine cyanobacterium Geitlerinema sp.

Nelson H Caicedo, Jolanta Kumirska, Jennifer Neumann, Stefan Stolte, Jorg Thöming

Index: Mar. Biotechnol. 14(4) , 436-45, (2012)

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Abstract

Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveries of the active compounds, a prerequisite for properly determining their concentration, are quantified here for the first time. We demonstrate a new procedure using Amberlite XAD-1180 resin in combination with the eluent isopropanol for extraction of the culture media and gas chromatography as simplified chemical analysis. This procedure reduced necessary bacteria cultivation time (from 150 to 21 days) at low volumes of culture media (300 mL) required for identification of two selected bioactive compounds: 4,4'-dihydroxybiphenyl and harmane.