Analytical Sciences 2003-01-01

Determination of trace amounts of bisphenol A in urine by negative-ion chemical-ionization-gas chromatography/mass spectrometry.

Tadashi Tsukioka, John Brock, Sam Graiser, Johnny Nguyen, Hiroyuki Nakazawa, Tsunehisa Makino

Index: Anal. Sci. 19(1) , 151-3, (2003)

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Abstract

We improved an analytical method for determining trace amounts of bisphenol A (BPA) in urine. BPA was subjected to enzymolysis and then to solid phase extraction with a C18 cartridge. The extract was eluted with methanol, and the eluate was concentrated under a nitrogen stream, and then pentafluorobenzylized in an alkali solution. The obtained pentafluorobenzylized compound was purifed using a florisil cartridge, followed by a determination using NCI-GC/MS. This method exhibited an excellent selectivity and reproducibility with a determination limit of 0.1 ng/ml.