Applied and Environmental Microbiology 2010-03-01

Efficient production of 2-oxobutyrate from 2-hydroxybutyrate by using whole cells of Pseudomonas stutzeri strain SDM.

Chao Gao, Wen Zhang, Chuanjuan Lv, Lixiang Li, Cuiqing Ma, Chunhui Hu, Ping Xu

Index: Appl. Environ. Microbiol. 76(5) , 1679-82, (2010)

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Abstract

2-Oxobutyrate is an important intermediate in the chemical, drug, and food industries. Whole cells of Pseudomonas stutzeri SDM, containing NAD-independent lactate dehydrogenases, effectively converted 2-hydroxybutyrate into 2-oxobutyrate. Under optimal conditions, the biocatalytic process produced 2-oxobutyrate at a high concentration (44.4 g liter(-1)) and a high yield (91.5%).