Biochemical and Biophysical Research Communications 2012-10-19

β-Adrenergic receptor signaling increases NAADP and cADPR levels in the heart.

Alexander M Lewis, Parvinder K Aley, Ali Roomi, Justyn M Thomas, Roser Masgrau, Clive Garnham, Katherine Shipman, Claire Paramore, Duncan Bloor-Young, Luke E L Sanders, Derek A Terrar, Antony Galione, Grant C Churchill

Index: Biochem. Biophys. Res. Commun. 427(2) , 326-9, (2012)

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Abstract

Evidence suggests that β-Adrenergic receptor signaling increases heart rate and force through not just cyclic AMP but also the Ca(2+)-releasing second messengers NAADP (nicotinic acid adenine dinucleotide phosphate) and cADPR (cyclic ADP-ribose). Nevertheless, proof of the physiological relevance of these messengers requires direct measurements of their levels in response to receptor stimulation. Here we report that in intact Langendorff-perfused hearts β-adrenergic stimulation increased both messengers, with NAADP being transient and cADPR being sustained. Both NAADP and cADPR have physiological and therefore pathological relevance by providing alternative drug targets in the β-adrenergic receptor signaling pathway.Copyright © 2012 Elsevier Inc. All rights reserved.