China Journal of Chinese Materia Medica 2012-12-01

[Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus].

Gui-Xiang Wan, Lin Ma, Jian Zhang

Index: Zhongguo Zhong Yao Za Zhi 37(24) , 3799-802, (2012)

Full Text: HTML

Abstract

To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions: ODS column was adopted, with methanol-water-phosphate (47: 53: 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.S. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).The experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.