PLoS ONE 2015-01-01

Organelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling.

Jianhua Wang, Yuzheng Zhao, Chao Wang, Qian Zhu, Zengmin Du, Aiguo Hu, Yi Yang

Index: PLoS ONE 10 , e0123986, (2015)

Full Text: HTML

Abstract

Nitric oxide (NO) is a membrane-permeable signaling molecule that is constantly produced, transferred, and consumed in vivo. NO participates and plays important roles in multiple biological processes. However, spatiotemporal imaging of NO in living cells is challenging. To fill the gap in currently used techniques, we exploited the versatility of HaloTag technology and synthesized a novel organelle-targetable fluorescent probe called HTDAF-2DA. We demonstrate the utility of the probe by monitoring subcellular NO dynamics. The developed strategy enables precise determination of local NO function.