Methods in Molecular Biology 2013-01-01

Application of dual cyclodextrin systems in capillary electrophoresis enantioseparations.

Anne-Catherine Servais, Marianne Fillet

Index: Methods Mol. Biol. 970 , 289-95, (2013)

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Abstract

The enantioseparation of acidic and neutral compounds can be successfully achieved in capillary electrophoresis (CE) using dual cyclodextrin (CD) systems. This chapter describes how to separate the enantiomers of acidic or neutral substances using dual CD systems made up of a negatively charged CD derivative, i.e., sulfobutyl-β-CD (SB-β-CD) or carboxymethyl-β-CD (CM-β-CD), in combination with a neutral one, namely, heptakis(2,3,6-tri-O-methyl)-β-CD (TM-β-CD). An acidic compound (carprofen) and a weakly acidic drug (pentobarbital) were selected as model compounds.