FEBS Letters 2015-10-07

Biophysical characterization of the interaction between FAAP20-UBZ4 domain and Rev1-BRCT domain.

Kyungeun Lim, Mi-Kyung Lee, Phuong T M Duong, Dinan Liu, Sieun Sung, Byong-Seok Choi

Index: FEBS Lett. 589 , 3037-43, (2015)

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Abstract

FAAP20 (Fanconi anemia-associated protein 20) is a subunit of the Fanconi anemia (FA) core complex that repairs interstrand cross-links. To understand the molecular basis for the FA core complex-mediated recruitment of Rev1 to the DNA lesion, we characterized the interactions among FAAP20-UBZ4, Rev1-BRCT, and ubiquitin using NMR. We found that FAAP20-UBZ4 binds not only ubiquitin but also Rev1-BRCT. Mapping the protein-protein interactions showed that FAAP20-UBZ4 has distinct binding surfaces for ubiquitin and Rev1-BRCT. In addition, the chemical exchange patterns indicated that the interaction between FAAP20-UBZ4 and ubiquitin might enhance the binding affinity between FAAP20-UBZ4 and Rev1-BRCT. These results provide new insight into the Rev1 recognition mechanism by FAAP20. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.