Guang pu xue yu guang pu fen xi = Guang pu 2010-03-01

[Study on the mechanism of Ca2+-buffering by sodium glycodeoxycholate micelles].

Hu-He Chen, Fang-Ting Yu, Zhan-Lan Yang, Yi-Zhuang Xu, Shi-Fu Weng, Jin-Guang Wu

Index: Guang Pu Xue Yu Guang Pu Fen Xi 30 , 748, (2010)

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Abstract

In the present article, NaGDC was used to study the Ca(2+)-buffering capability of bile salt micelles. NaGDC is a naturally occurring bile salt which constitutes approximately 10 molar percent of bile salt composition in gallbladder. The authors selected glycine conjugated bile salts NaGDC because it precipitates with Ca2+ ions fast. The Ca(2+)-buffering property of sodium glycodeoxycholate (NaGDC) micelles was studied by utilizing turbidity titration, quasi-elastic light scattering (QELS), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). When the concentration of NaGDC was above the CMC, the precipitation of Ca2+ ions with NaGDC was buffered. The Ca(2+)-buffering capability was enhanced when the concentration of NaGDC increased. The possible mechanism of Ca(2+)-buffering by NaGDC micelles was discussed. When the concentration of NaGDC was below the CMC, the Ca2+ ions reacted with GDC-anions to form the Ca(GDC)2 precipitation. However, at the concentration above the CMC, NaGDC simple micelles were connected by Ca(2+)-bridge to form larger fibriform micelles via the interaction of carboxyl groups of NaGDC simple micelles with Ca2+ ions. When the ratio of Ca2+ to Na+ was enhanced to the fixed value, Na(n)Ca(m) (GDC)(n+2m) complexes were precipitated by the further addition of Ca2+ ions.