Journal of chromatography. B, Biomedical applications 1995-10-20

Automated screening system for purine and pyrimidine metabolism disorders using high-performance liquid chromatography.

S Sumi, K Kidouchi, S Ohba, Y Wada

Index: J. Chromatogr. B, Biomed. Appl. 672(2) , 233-9, (1995)

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Abstract

An automated screening system for purine and pyrimidine metabolism disorders using high-performance liquid chromatography (HPLC) with column switching is described. The system consists of a reversed-phase column, a cation-exchange column, a column switch, four sets of ultraviolet absorbance detectors, a microcomputer and other conventional equipment. As this system permits the simultaneous determination of urinary orotic acid, uracil, dihydrouracil, pseudouridine, xanthine, 2,8-dihydroxyadenine and succinyladenosine, it offers a useful method for the detection of orotic aciduria, dihydropyrimidine dehydrogenase deficiency, dihydropyrimidinuria, xanthinuria, adenine phosphoribosyltransferase deficiency and adenylosuccinase deficiency.