International Journal of Biological Macromolecules 2015-01-01

Alginate-based hydrogels with improved adhesive properties for cell encapsulation.

Bapi Sarker, Julia Rompf, Raquel Silva, Nadine Lang, Rainer Detsch, Joachim Kaschta, Ben Fabry, Aldo R Boccaccini

Index: Int. J. Biol. Macromol. 78 , 72-8, (2015)

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Abstract

Hydrogel-based biomaterials are ideal scaffolding matrices for microencapsulation, but they need to be modified to resemble the mechanical, structural and chemical properties of the native extracellular matrix. Here, we compare the mechanical properties and the degradation behavior of unmodified and modified alginate hydrogels in which cell adhesive functionality is conferred either by blending or covalently cross-linking with gelatin. Furthermore, we measure the spreading and proliferation of encapsulated osteoblast-like MG-63 cells. Alginate hydrogels covalently crosslinked with gelatin show the highest degree of cell adhesion, spreading, migration, and proliferation, as well as a faster degradation rate, and are therefore a particularly suitable material for microencapsulation. Copyright © 2015 Elsevier B.V. All rights reserved.