Angewandte Chemie. International edition in English 2012-10-29

Heterologous expression and manipulation of three tetracycline biosynthetic pathways.

Peng Wang, Woncheol Kim, Lauren B Pickens, Xue Gao, Yi Tang

Index: Angew. Chem. Int. Ed. Engl. 51(44) , 11136-40, (2012)

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Abstract

A very accommodating host: Three tetracycline biosynthetic pathways were overexpressed and manipulated in the heterologous host Streptomyces lividans K4-114. Through the inactivation of various genes and characterization of the resulting biosynthetic intermediates, new tetracycline-modifying enzymes were identified (see scheme).Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.