Drug and Chemical Toxicology 2015-01-01

Limoniastrum guyonianum methanol extract induces immunomodulatory and anti-inflammatory effects by activating cellular anti-oxidant activity.

Mounira Krifa, Nadia Mustapha, Zied Ghedira, Kamel Ghedira, Antonio Pizzi, Leila Chekir-Ghedira

Index: Drug Chem. Toxicol. 38(1) , 84-91, (2015)

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Abstract

Evaluation of the immunomodulatory activity of plant extracts is an interesting and growing area of research. In this study, effects of a methanolic extract of Limoniastrum guyonianum stems (M extract) on mice immune cell function in vitro were investigated. These studies showed that mitogen-induced lymphocyte proliferation was dose-dependently inhibited by the extract. Further, the lectin-induced response appeared to be more sensitive to the suppressive effects of the extract than were LPS-stimulated responses. In studies to assess any potential effects of extract on innate immunity, the results showed that the extract significantly enhanced the killing activity of isolated NK cells. In addition, studies here demonstrated that the extract could enhance lysosomal enzyme activity and inhibit nitrite oxide (NO) production by murine peritoneal macrophages ex vivo, suggesting a potential anti-inflammatory effect in situ. The anti-inflammatory activity was concomitant with the cellular anti-oxidant effect in macrophages and splenocytes.