Reproductive Toxicology 2015-08-15

Incubation at 32.5°C and above causes malformations in the zebrafish embryo.

Casper Pype, Evy Verbueken, Moayad A Saad, Christophe R Casteleyn, Chris J Van Ginneken, Dries Knapen, Steven J Van Cruchten

Index: Reprod. Toxicol. 56 , 56-63, (2015)

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Abstract

Zebrafish embryos are increasingly used for developmental toxicity screening of candidate drugs and are occasionally co-incubated with a metabolic activation system at 32°C for 1, 2 or 4h, depending on their developmental stage. As this temperature is higher than the optimal temperature for zebrafish embryonic development (26-28.5°C), we investigated whether continuous incubation of zebrafish embryos from 2.5 until 96h post fertilization (hpf) at high temperatures (30.5-36.5°C) causes malformations. At 32.5°C tail malformations were observed as early as 24hpf, and these became even more prominent at 34.5 and 36.5°C. Cardiovascular and head malformations, edema and blood accumulations throughout the body were present at 36.5°C. Finally, temperatures higher than 28.5°C accelerated embryonic development except for 36.5°C, at which a lower hatching rate and hatching enzyme activity were observed. In conclusion, incubation of zebrafish embryos at 32.5°C and above from 2.5 until 96hpf causes malformations as early as 24hpf. Copyright © 2015 Elsevier Inc. All rights reserved.