Analytical Biochemistry 2014-07-01

Coordination complexes as molecular glue for immobilization of antibodies on cyclic olefin copolymer surfaces.

Huey Wen Ooi, Shaun J Cooper, Chang-Yi Huang, Dean Jennins, Emma Chung, N Joe Maeji, Andrew K Whittaker

Index: Anal. Biochem. 456 , 6-13, (2014)

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Abstract

A novel metal-based chelating method has been used to provide an order of magnitude increase in immunoassay performance on cyclic olefin copolymer (COC) plastics compared with passive binding. COCs are hydrophobic, and without surface modification they are often unsuitable for applications where protein adhesion is desired. When interacting with the bare plastic, the majority of the bound proteins will be denatured and become nonfunctional. Many of the surface modification techniques reported to date require costly equipment setup or the use of harsh reaction conditions. Here, we have successfully demonstrated the use of a simple and quick metal chelation method to increase the sensitivity, activity, and efficiency of protein binding to COC surfaces. A detailed analysis of the COC surfaces after activation with the metal complexes is presented, and the immunoassay performance was studied using three different antibody pairs. Copyright © 2014 Elsevier Inc. All rights reserved.