Preparation of high specific activity tritium??labelled leukotriene B4 suitable for radioligand binding assay

SI Schramm, IY Nagaev, A Sabirsh…

Index: Journal of Labelled Compounds and Radiopharmaceuticals, , vol. 51, # 2 p. 101 - 105

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Abstract

... Figure 3. Separation of tritium-labelled LTB4 and 5,12-diHETE by RP-HPLC in an acetonitrile-based solvent system. ... J. Label Compd. ... with LTB4 binding to BLT1.15 Taken together these results demonstrate that the [3H] LTB4 prepared is useful for labelling LTB4 receptors ...