Biological and pharmaceutical bulletin

Appearance of Compound K, a Major Metabolite of Ginsenoside Rb1 by Intestinal Bacteria, in Rat Plasma after Oral Administration. Measurement of Compound K by …

T Akao, M Kanaoka, K Kobashi

Index: Akao, Teruaki; Kanaoka, Matao; Kobashi, Kyoichi Biological & Pharmaceutical Bulletin, 1998 , vol. 21, # 3 p. 245 - 249

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Citation Number: 177

Abstract

Enzyme immunoassay (EIA) for the determination of compound K (CK), a major metabolite of ginsenoside Rb 1 (G-Rb 1) from Panax ginseng root by intestinal bacterial flora, was explored. Bovine serum albumin (BSA) was coupled to the C-26 position on the unsaturated side chain of CK. β-D-Galactosidase was introduced at the C-26 position of the saturated side chain. Antiserum, obtained by immunization of rabbits with CK-BSA conjugate, ...