Development of an enzyme-linked immunosorbent assay for the determination of the linear alkylbenzene sulfonates and long-chain sulfophenyl carboxylates using …

J Ramón-Azcón, R Galve, F Sánchez-Baeza…

Index: Ramon-Azcon, Javier; Galve, Roger; Sanchez-Baeza, Francisco; Marco, M.-Pilar Analytical Chemistry, 2006 , vol. 78, # 1 p. 71 - 81

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Citation Number: 24

Abstract

ELISA methods have been developed for screening contamination of water resources by linear alkyl benzene sulfonates (LAS) or the most immediate degradation products, the long chain sulfophenyl carboxylates, SPCs. The assay uses antibodies raised through pseudoheterologous immunization strategies using an equimolar mixture of two immunogens (SFA-KLH and 13C13-SPC-KLH) prepared by coupling N-(4-alkylphenyl) ...