Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis

…, I Casabon, R Gruninger, NC Strynadka…

Index: Capyk, Jenna K.; Casabon, Israel; Gruninger, Robert; Strynadka, Natalie C.; Eltis, Lindsay D. Journal of Biological Chemistry, 2011 , vol. 286, # 47 p. 40717 - 40724

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Citation Number: 35

Abstract

↵3 To whom correspondence should be addressed: 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada. Tel.: 604-822-0042; Fax: 604-822-6041; E-mail: leltis{at}mail.ubc.ca. ... ↵1 These authors contributed equally to this work. ... Background: Mycobacterium tuberculosis (Mtb) degrades cholesterol throughout its infection cycle. ... Results: Cholesterol ring-degrading enzymes have higher activities with side chain degradation intermediates ...