4-(7-Diethylaminocoumarin-3-yl) benzoyl cyanide (DACB-CN) was synthesized as a new fluorescent derivatization reagent for alcohols for use in high-performance liquid chromatography. Saturated alcohols (C 8-C 22) were derivatized in good yields to give the corresponding fluorescent DACB-esters by treatment with DACB-CN. The DACB-esters of these alcohols were clearly separated on a reversed-phase HPLC column. The detection ...
