Tetrahedron letters

The development of a fluorescence turn-on sensor for cysteine, glutathione and other biothiols. A kinetic study

O García-Beltrán, N Mena, EG Pérez, BK Cassels…

Index: Garcia-Beltran, Olimpo; Mena, Natalia; Perez, Edwin G.; Cassels, Bruce K.; Nunez, Marco T.; Werlinger, Francisca; Zavala, Daniel; Aliaga, Margarita E.; Pavez, Paulina Tetrahedron Letters, 2011 , vol. 52, # 49 p. 6606 - 6609

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Citation Number: 22

Abstract

Two fluorescence probes for the detection of cysteine (Cys), glutathione (GSH) and other biothiols, such as homocysteine (Hcy) and cysteinyl-glycine (Cys-Gly), were developed. These molecular probes are coumarin-based derivatives containing a chalcone-like moiety that reacts with biothiols through a Michael addition reaction, leading to strong fluorescence enhancements. The reactivity of the tested biothiols toward both probes (ChC1 and ChC2) ...