Synthesis of 1-Piperideine-6-carboxylic Acid Produced by l-lysine-ε-aminotransferase from the Streptomyces clavuligerus Gene Expressed in Escherichia coli

ML Wu, JH Chen, CT Ho, TC Huang

Index: Wu, Mei-Li; Chen, Jun-Hao; Ho, Chi-Tang; Huang, Tzou-Chi Journal of Agricultural and Food Chemistry, 2007 , vol. 55, # 5 p. 1767 - 1772

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Citation Number: 8

Abstract

The gene (lat) encoding l-lysine ε-aminotransferase (LAT) in Streptomyces clavuligerus was cloned and expressed in Escherichia coli. Nucleotide sequence analysis of lat predicted a single open reading frame (ORF) of 1371 bp, encoding a polypeptide of 457 amino acids with calculated molecular mass of 49.89 kDa. S. clavuligerus LAT was grouped into aminotransferase subfamily II of α family on the basis of sequence homology. A model ...