Substrate specificity of glycyrrhizinic acid hydrolase.

Y Sasaki, T Morita, T Kuramoto, K MIZUTANI…

Index: Sasaki, Yasuhiro; Morita, Toshinobu; Kuramoto, Takashi; Mizutani, Kenji; Ikeda, Ryuko; Tanaka, Osamu Agricultural and Biological Chemistry, 1988 , vol. 52, # 1 p. 207 - 210

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Citation Number: 22

Abstract

Glycyrrhizinic acid hydrolase produced by Aspergillus niger selectively hydrolyzed the 3-O-β- D-glucuronide linkage of glycyrrhizinic acid. The substrate specificity of this enzyme was investigated for synthetic glucuronides of aliphatic alcohols as well as natural glucuronide saponins. It was revealed that the glucuronide linkage with low molecular weight alcohols was not cleaved by this enzyme, while the 3-O-β-D-glucuronide linkage of saponins of ...