Journal of the American Chemical Society

Biosynthesis of a site-specific DNA cleaving protein

HS Lee, PG Schultz

Index: Hyun, Soo Lee; Schultz, Peter G. Journal of the American Chemical Society, 2008 , vol. 130, # 40 p. 13194 - 13195

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Citation Number: 51

Abstract

An E. coli catabolite activator protein (CAP) has been converted into a sequence-specific DNA cleaving protein by genetically introducing (2, 2′-bipyridin-5-yl) alanine (Bpy-Ala) into the protein. The mutant CAP (CAP-K26Bpy-Ala) showed comparable binding affinity to CAP- WT for the consensus operator sequence. In the presence of Cu (II) and 3- mercaptopropionic acid, CAP-K26Bpy-Ala cleaves double-stranded DNA with high ...

~75%

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