Turnover is rate-limited by deglycosylation for Micromonospora viridifaciens sialidase-catalyzed hydrolyses: conformational implications for the Michaelis complex

J Chan, A Lu, AJ Bennet

Index: Chan, Jefferson; Lu, April; Bennet, Andrew J. Journal of the American Chemical Society, 2011 , vol. 133, # 9 p. 2989 - 2997

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Citation Number: 17

Abstract

A panel of seven isotopically substituted sialoside natural substrate analogues based on the core structure 7-(5-acetamido-3, 5-dideoxy-d-glycero-α-d-galacto-non-2-ulopyranosylonic acid)-(2→ 6)-β-d-galactopyranosyloxy)-8-fluoro-4-methylcoumarin (1, Neu5Acα2, 6GalβFMU) have been synthesized and used to probe the rate-limiting step for turnover by the M. viridifaciens sialidase. The derived kinetic isotope effects (KIEs) on k cat for the ring ...