| 常用名 | 核糖核酸酶T1 来源于米曲霉(色谱纯化) | CAS号 | 9026-12-4 |
|---|---|---|---|
| 价格 | ¥需询单/100g ¥需询单/500g | 纯度 | 98.0% |
| 备货期 | 需询单 | 库存 | 现货 |
| 产品详情(用途,包装等)
用途: Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA. 物理化学性质: CAS号:9026-12-4 常用中文名:核糖核酸酶T1 来源于米曲霉(色谱纯化) 常用英文名:Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.) 分子式: 分子量:384.41 密度:N/A 沸点:N/A 熔点:N/A 闪点:N/A 储存条件:2-8°C |