Analytical Biochemistry 1984-10-01

Construction of a microprocessor-controlled pulsed quench-flow apparatus for the study of fast chemical and biochemical reactions.

J Langowski, C Urbanke, E Schuppe

文献索引：Anal. Biochem. 142(1) , 91-7, (1984)

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摘要

The construction and operation of a microprocessor-controlled quenched-flow machine are described. Two sets of syringes are moved by high-torque stepping motors to achieve any desired mixing scheme. The dead time of the instrument is pulsed quench-flow operation is of the order of 10 ms. The fastest possible reaction time is 12 ms with the reaction chamber used here; this might be extended downward with other reaction chambers. The reactant volume required is low; 1 ml of solution for each reactant is sufficient for more than 40 kinetic measurements. The machine is tested by alkaline hydrolysis of 2,4-dinitrophenylacetate and used in the investigation of the reaction mechanism of the EcoRI restriction endonuclease and of GTPase activity of ribosomes.