Clinica Chimica Acta 2001-12-01

Measurement of serum digitoxin in patients by radioimmunoassay using specific antiserum.

Y Ikeda, M Fujii, M Yamazaki, Y Fujii

文献索引：Clin. Chim. Acta 314(1-2) , 245-7, (2001)

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摘要

Digitoxin is used to treat patients with heart failure.A radioimmunoassay procedure for the specific determination for digitoxin in serum was developed using the antiserum (antiserum (A)) raised against digitoxin 3'-hemisuccinate-BSA conjugate.The intra- and interassay variability were <10% in the range of 5-100 ng/ml. The specificities of antiserum (A) and the commercial anti-digitoxin antiserum (antiserum (B)) were assessed by cross-reactivity studies with various related compounds. Antiserum (A) was highly specific for digitoxin. Mean digitoxin concentrations in serum samples (n=34) from digitalized patients by RIA using these antisera were 10.0 and 12.4 ng/ml, respectively.This RIA using antiserum (A) measure unmetabolized digitoxin and may be applicable for pharmacokinetic studies.