Tetrahedron

Synthesis of immunocomponents for the measurement of lead (Pb) by fluorescence polarization immunoassay

M Adamczyk, JR Fishpaugh, KJ Heuser, JM Ramp…

文献索引：Adamczyk, Maciej; Fishpaugh, Jeffrey R.; Heuser, Kevin J.; Ramp, John M.; Reddy, Rajarathnam E.; Wong, Martin Tetrahedron, 1998 , vol. 54, # 13 p. 3093 - 3112

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被引用次数: 20

摘要

Three novel haptens (5b, 9b, 11b) were synthesized from porphobilinogen (2) and conjugated to bovine serum albumin to afford immunogens (6, 10, 12). Five fluorescent tracers (14, 17, 19, 29, 32) were prepared from porphobilinogen (2), or haptens (9b, 11b, 20b) in> 98% purity. the immunogens (6, 10, 12) were utilized for generation of polyclonal antibodies and the best tracer-antibody combination was selected to construct the ...