Bioorganic & Medicinal Chemistry Letters 1998-05-05

Synthesis of 14 C-radiolabeled oligonucleotides with a novel phosphoramidite reagent.

A Guzaev, B Boyode, G Balow, K L Tivel, M Manoharan

文献索引：Bioorg. Med. Chem. Lett. 8(9) , 1123-6, (1998)

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摘要

A novel solid-phase synthesis of 5'-radiolabeled oligonucleotides is described. The labeling reaction is carried out by the phosphoramidite method with the aid of [4,6-di-14C]-5'-dimethoxytritylthymidine building block 1. The feasibility of the method is demonstrated by preparation of 3'-phosphorylated dodecathymidylate phosphorothioate containing radiolabeled nucleoside at the 5'-terminus.